Publications Citing Cellvis (formerly In Vitro Scientific) Products
51 - 100 of 577 publications
Protein oligomerization and mobility within the nuclear envelope evaluated by the time-shifted mean-segmented Q factor
J Hennen, et al., Methods Available online 28 September 2018
Quote: "Experiments were performed using transiently transfected U2OS cells (ATCC, Manassas, VA), which were maintained in DMEM with 10% FBS (Hyclone Laboratories, Logan, UT) before being sub-cultured into 24-well glass bottom plates (In Vitro Scientific, Sunnyvale, CA) 24 h"
Fast dynamic in vivo monitoring of Erk activity at single cell resolution in DREKA zebrafish
V Mayr, et al., Front Cell Dev Biol. 2018; 6: 111.
Quote: "A2576-25G, Sigma-Aldrich Chemie GmbH, Steinheim, Germany) in a glass bottom imaging dish (D35-14-1.5-NJ, Cellvis, Mountain View, CA, USA). Images and time-lapse movies were recorded on a Leica SP8 X WLL confocal microscope system"
A polymer-free, biomimicry drug self-delivery system fabricated via a synergistic combination of bottom-up and top-down approaches
X Xu, et al., J of Materials Chem. Issue 47, 2018
Quote: "The A549 lung cancer cells were seeded into glass-bottom dishes (Cellvis, Mountain View, CA) and treated with 25 μM RBC-PI."
Microtubules Gate Tau Condensation to Spatially Regulate Microtubule Functions
Ruensern Tan, et al., BioRxiv, September 22, 2018
Quote: "Continuous imaging assays: Tau condensation assays (Fig. 1A-C, S1A) were conducted in Cellvis 96-well Glass Bottom Plate (Cellvis, #P96-1.5H-N) as previously described"
Physicochemical mechanotransduction alters nuclear shape and mechanics via heterochromatin formation
AD Stephens, et al., BioRxiv September 21, 2018
Quote: "Live cell imaging: Cells were grown to the desired confluence in cell culture dishes containing glass coverslip bottoms (In Vitro Scientific)"
Characterization of Endothelial Cilia Distribution During Cerebral-Vascular Development in Zebrafish (Danio rerio)
S Eisa-Beygi, et al., ATVB Vol 38, Issue 12, 2018
Quote: "Embryos were mounted on 35 mm glass-bottom Petri dishes (CELLVIS) and multiple Z-stacks were taken with a Zeiss LSM 510 AxioImager."
Inhibitor experiments Fresh isolated human neutrophils (10,000 per well) were seeded in 96-glassbottom-well-plates (In vitro scientific) and activated for NETosis with PMA, final concentration 100 nM
Elsa Neubert, et al., Nature Communications volume 9, Article number: 3767 (2018)
Quote: "Inhibitor experiments: Fresh isolated human neutrophils (10,000 per well) were seeded in 96-glassbottom-well-plates (In vitro scientific) and activated for NETosis with PMA, final concentration 100 nM"
Visualization of Calcium Ion Loss from Rotavirus during Cell Entry
Eric N. Salgado, et al., Journal of Virology, 2018
Quote: "For live-cell imaging, BSC-1 cells were grown on 8-chambered number 1.5 cover glass slides (Cellvis) in a volume of 400 μl supplemented DMEM and allowed to grow overnight to 50% confluence."
Systems-level properties of EGFR/Ras/ERK signalling amplify local signals to generate gene expression plasticity
AE Davies, et al., BioRxiv November 09, 2018
Quote: "Live-cell microscopy and co-culture conditions: Live-cell microscopy was conducted on multi-well plates with #1.5 glass bottoms (In Vitro Scientific, Mountain View, CA) that were coated with laminin-111 (Invitrogen, Carlsbad, CA). 50ug/ml Laminin-111 was deposited"
Effects of bisphenol A on gap junctions in HaCaT cells as mediated by the estrogen receptor pathway
Q Zhang, et al., Journal of Applied Toxicology, 06 September 2018
Quote: "The single cell suspension was prepared and seeded in 96‐well plates (for evaluation of cell proliferation, 100 μL/well), or glass bottom dishes (for evaluation of GJIC, 2 mL/ dish, 35 mm; In Vitro Scientific, Mountain View, CA, USA)"
A High Avidity Biosensor Reveals PI (3, 4) P2 is Predominantly a Class I PI3K Signaling Product
B Goulden, et al., bioRxiv, September 06, 2018
Quote: "For transfection, cells were seeded in 35 mm tissue culture dishes with 20 mm Number 1.5 cover glass apertures (CellVis) coated with 5 µg fibronectin (Life Technologies 33016-015)"
Spatial Proliferation of Epithelial Cells Is Regulated by E-Cadherin Force
A Mohan, et al., Biophysical Jopurnal, Volume 115, Issue 5, 4 September 2018, Pages 853-864
Quote: "FRET imaging and analysis. Cells were seeded onto a 35 mm dish with a 1.5 NA (numerical aperture) 20-mm glass-bottom coverslip dishes (Cellvis, Sunnyvale, CA) that were precoated with 40 μg/mL fibronectin"
3D Bioprinting–Flow Cytometry as Analytical Strategy for 3D Cell Structures
S Gretzinger, et al., Bioprinting Volume 11, September 2018
Quote: "Round scaffolds of 9 mm in diameter and 4 horizontal and vertical lines (line spacing 2.25 mm) were printed up to a height of 3 mm directly into 6 well glass bottom plates (Cellvis, Mountain View, USA)"
Cartilage tissue formation through assembly of microgels containing mesenchymal stem cells
F Li, VX Truong, et al., Acta Biomaterialia Volume 77, 1 September 2018, Pages 48-62
Quote: "1% (w/v) PEGdiSH, 0.03% (w/v) LAP and 2.5 million cells mL−1 were mixed thoroughly and 100 µL was pipetted into the petri dish (Cellvis, USA)."
F-actin dynamics transform filopodial bridges into intercellular nanotubes capable of distant cell communication
Minhyeok Chang, et al., bioRxiv, August 31, 2018
Quote: "After the supernatants were removed, the cells were suspended in different volumes of medium in a poly(dimethylsiloxane) (PDMS) chamber for live cell imaging52 or a 4-chamber glass bottom dish (Cellvis, fixed cell or live cell imaging) to control the cell density."
A FRET-Based approach to study the SUMOylation of Serotonin 1A Receptors
S Kaur, et al., Ku Schoolworks, 2018
Quote: "or in 96-well glass bottom plates (Catalog no. #P96-1-N, CellVis, Mountain View, CA, USA) at a seeding density of 15,000 cells per well."
a novel multifunctional anti-cea-il15 molecule displays potent antitumor activities
Y Liu, et al., Drug Des Devel Ther. 2018; 12: 2645–2654.
Quote: "Immunofluorescence assay. SKOV3 and LS174T cells were cultured in 30 mm glass bottom dishes (Cellvis, Mountain View, CA, USA) to 70% confluence"
Development of a Novel 3D Tumor-tissue Invasion Model for High-throughput, High-content Phenotypic Drug Screening
T. J. Puls, et al., Nature: Scientific Reports, volume 8, Article number: 13039 (2018)
Quote: "Overall dimensions and post spacing were optimized to accommodate glass-bottom 96-well plates (Cellvis, Mountain View, CA; Supplemental Fig. S1)."
Investigating LINC complex protein homo-oligomerization in the nuclear envelopes of living cells using fluorescence fluctuation spectroscopy
Jared Hennen, et al., Methods in Molecular Biology, The LINC Complex pp 121-135
Quote: "The FFS experimental setup used in this work. 2.2 Samples and Microscope Slide. 1. 24-well glass-bottom slide with #1.5H cover glass (In Vitro Scientific, Sunnyvale, CA)"
Using Nesprin Tension Sensors to Measure Force on the LINC Complex
PT Arsenovic, et al., The LINC Complex pp 59-71
Quote: "For all imaging experiments, cells are grown on #1.5 coverglass bottom dishes (Cellvis D35-20-1.5-N)."
Frequency-encoded multicolor fluorescence imaging with single-photon-counting color-blind detection
ET Garbacik, et al., Biophysical Journal, Volume 115, Issue 4, 21 August 2018, Pages 725-736
Quote: "HeLa-MannII-GFP cells were seeded on a 35-mm glass-bottomed dish with 20-mm microwell #1 cover (Cellvis, Mountain View, CA) 24 h before fixation"
Clinical lymphocytes construction for light scattering inversion study: a three-dimensional morphology constructed method from defective confocal images
L Zhang, et al., J. of Biomedical Optics, 23(8), 085003 (2018).
Quote: "To ensure spatial stability of lymphology during fluorescence confocal imaging, a 20 to 30 μ L drop is dripped on to a 60-mm glass bottom dish with a 14-mm bottom well (In Vitro Scientific Co., Ltd.)"
Functional analysis of a hypomorphic allele shows that MMP14 catalytic activity is the prime determinant of the Winchester syndrome phenotype
IJHM de Vos, et al., Human Molecular Genetics, Volume 27, Issue 16, 15 August 2018, Pages 2775–2788
Quote: "In brief, a 4-chamber glass bottom dish (Cellvis, Mountain View, California, USA; D35C4-20-1.5-N) was coated with poly-L-Lysine, glutaraldehyde and Cy3-labelled gelatin in sequence"
A Nanomicellar Prodrug Carrier Based on Ibuprofen-Conjugated Polymer for Co-delivery of Doxorubicin
Z Li, et al., Front Pharmacol. 2018; 9: 781.
Quote: "Intracellular Trafficking. 4T1.2 cells (15,000/well) were seeded in glass bottom dishes (In Vitro Scientific, United States), and incubated overnight."
Mass spectrometry‐based proteomic analysis reveals the interacting partners of lipin1
J Wang, et al., IUBMB, Volume70, Issue8 August 2018
Quote: "For immunofluorescence, cells were plated on 4 chamber glass bottom dishes (Cellvis, CA, USA) and trans- fected with pcDNA3.1/myc-His A-lipin1 for 48 h."
Bimolecular Fluorescence Complementation Methodology to Study G Protein-Coupled Receptor Dimerization in Living Cells
D Alongkronrusmee, et al., Receptor-Receptor Interactions in the Central Nervous System pp 205-221
Quote: "Four chamber 35 mm glass-bottom dish (Cellvis, Mountain View, CA, USA) for a confocal microscopic detection"
Pooled optical screens in human cells
D Feldman, et al., BioRxiv, August 02, 2018
Quote: "Cells were then split for screening either via in situ sequencing or by FACS. For in situ screening, 500,000 cells were seeded into each well of a glass-bottom 6-well plate (CellVis)."
Induction of oxidative stress by long-term treatment of live HEK293 cells with therapeutic concentration of lithium is associated with down-regulation of δ-opioid receptor amount and function
M Vosahlikova, et al., Biochemical Pharmacology Volume 154, August 2018, Pages 452-463
Quote: "The 35 mm glass bottom dish, dish size 35 mm, well size 20 mm, #1.5 cover glass (D35-20-1.5-N) was purchased from Cellvis (Mountain View, California, USA)"
Parallel High-Resolution Imaging of Leukocyte Chemotaxis Under Agarose with Rho-Family GTPase Biosensors
GRR Bell, et al., Rho GTPases pp 71-85, 2018
Quote: "Imaging Materials. 1. Glass-bottom 96-well plate (Cellvis, Catalog #P96–1.5HN or equivalent)"
Cell Polarity and Division Symmetry Analyses in Transformed Blood Cells
B Mizukawa, et al., Rho GTPases pp 257-266
Quote: "35 mm Glass-bottom dish (#1.5) with 10 mm diameter microwell (Cellvis D35-10-1.5-N)"
Ki67 is a Graded Rather than a Binary Marker of Proliferation versus Quiescence
I Miller, et al., Cell Reports, Volume 24, Issue 5, 31 July 2018, Pages 1105-1112
Quote: "96 well glass bottom plate, Cellvis, Cat# P96-1.5HN"
NF-κB directly regulates β-arrestin-1 expression and forms a negative feedback circuit in TNF-α–induced cell death
J Li, et al., FASEB Journal, 30 Jul, 2018
Quote: "Live cell imaging HEK293 cells were cultured in 35-mm glass-bottom dishes (In Vitro Scientific, Sunnyvale, CA, USA). Vectors that contained GFP-b-arrestin-1, GFP-b-arrestin-1 Q394L, or GFP-b-arrestin-2 were transfected into cells according to the calcium phosphate method"
Controllable protein phase separation and modular recruitment to form responsive membraneless organelles
Benjamin S. Schuster, et al., Nature Communications volume 9, Article number: 2985 (2018)
Quote: "HEK293, HeLa, and U2OS cells were plated at a density of 75 000 cells/well in 0.5 mL Dulbecco’s modified Eagle’s medium (supplemented with 10% fetal bovine serum, glutamine, and pen/strep) in a 24-well glass-bottom plate (#1.5 cover glass; Cellvis) and incubated using standard tissue culture methods at 37 °C with 5% CO2 and saturating humidity"
Delivery of Fluorescent Probes Using Streptolysin O for Fluorescence Microscopy of Living Cells
KW Teng, et al., Current Protocols, Volume93, Issue1 August 2018
Quote: "Adherent cells are typically subcultured on a 35‐mm glass‐bottom dish with 14‐mm microwells (eg, Cellvis, cat. no. D35‐14‐1‐N) 2 days before the permeabilization experiment"
A perfused human blood–brain barrier on-a-chip for high-throughput assessment of barrier function and antibody transport
Nienke R. Wevers, et al., Fluids and Barriers of the CNS201815:23
Quote: "TY10 cells grown in a collagen-I coated 24-well glass bottom plate (P24-0-N, Cellvis, Mountain View, CA, USA) were fixed with 4% paraformaldehyde"
Vertebrate Dynein-f depends on Wdr78 for axonemal localization and is essential for ciliary beat
Y Zhang, et al., Journal of Molecular Cell Biology, 28 July 2018
Quote: "The remaining radial glia-enriched cells were further cultured to ∼80% con- fluency (usually 3 days) and then transferred into the wells of laminin-coated 29-mm glass-bottom dishes (Cellvis, D29-14-1.5-N) at a density of 2 × 10 5 cells per well (SS d–3)"
3D visualization of extracellular vesicle uptake by endothelial cells
M Durak-Kozica, et al., Cellular & Molecular Biology Letters 2018 23:57
Quote: "For confocal microscopy observations, BIO-PORT glass bottom dishes (thickness #1.5) were purchased from Cellvis."
Mother centrioles are dispensable for deuterosome formation and function during basal body amplification
H Zhao, et al., BioRxiv, July 20, 2018
Quote: "The cells were then transferred into the wells of laminin-coated 29-mm glass-bottom dishes (Cellvis, D29-14-1.5-N) at a density of 2×105 cells per well and were maintained in serum-free medium to induce multiciliate mEPCs"
Chronic Electrical Stimulation Promotes the Excitability and Plasticity of ESC-derived Neurons following Glutamate-induced Inhibition In vitro
CFV Latchoumane, et al., Nature, Scientific Reportsvolume 8, Article number: 10957 (2018)
Quote: "Briefly, each well of 12 well/64 electrode per well containing MEA plates (Axion Biosystems, GA), or glass bottom petri dishes (Cellvis, CA) were coated with polyethylenimine (PEI) (Sigma-Aldrich, MO) and incubated for 1 h at 37 °C and 5% CO2"
Optimal fluorescent protein tags for quantifying protein oligomerization in living cells
Valentin Dunsing, et al., Nature: Scientific Reportsvolume 8, Article number: 10634 (2018)
Quote: "For microscopy experiments, 6 × 105 (HEK) or 4 × 105 (A549, CHO, HeLa, U2OS) cells were seeded in 35 mm dishes (CellVis, Mountain View, CA or MatTek Corp., Ashland, MA) with optical glass bottom, 24 h before transfection."
Nile Red derivatives enable improved ratiometric imaging for nerve-specific contrast
Jesse R. Korber, et al., J. of Biomedical Optics, 23(7), 076002 (2018).
Quote: "For imaging studies, cells were grown in 96-well glass bottom plates (Cellvis, Mountain View, California) for 48 h, with a final confluence of <80%."
Blue light excited retinal intercepts cellular signaling
Kasun Ratnayake, et al., Scientific Reportsvolume 8, Article number: 10207 (2018)
Quote: "For live cell imaging experiments, HeLa cells were cultured on 35 mm glass bottom dishes (In Vitro Scientific) with 1 × 105/mL cell density"
Near infrared fluorescent peptide nanoparticles for enhancing esophageal cancer therapeutic efficacy
Zhen Fan, et al., Nature Communications, volume 9, Article number: 2605 (2018)
Quote: "Confocal imaging for protein expression and localization: To verify the expression level of CD51, cells were seeded into glass-bottom dish with thickness of 0.16–0.19 mm (In vitro Scientific, USA)."
Add-on plasmonic patch as a universal fluorescence enhancer
J Luan, et al., Nature: Light: Science & Applicationsvolume 7, Article number: 29 (2018)
Quote: "Fluorescence-linked immunosorbent assay was first implemented using 96-well plates with a glass bottom (Cellvis). The glass surface of each well was treated to achieve aldehyde functionality"
Giardia's ventral disc is hyperstable and composed of over 80 disc-associated proteins
Christopher Nosala, et al., BioRxiv July 03, 2018.
Quote: "Confocal Microscopy: 3D stacks and time lapse movies were acquired of live cells grown in 96-well #1.5 black glass14 bottom imaging plates (In Vitro Scientific)."
StaPLs: versatile genetically encoded modules for engineering drug-inducible proteins
Conor L. Jacobs, et al., Nature Methods 2018
Quote: "Fluorescence widefield microscopy of live transfected HeLa cells (in 35-mm glass-bottom four chamber dishes; In Vitro Scientific), live or fixed transfected HEK293-TRE3GmCherry stable cells (in plastic 12-well plates (Greiner Bio-One) or glass-bottom 12-well plates (In Vitro Scientific))"
Data confirming murine erythrocyte opsonization and oxidative damage and live microscopic analysis of oxidatively damaged erythrocyte uptake by mast cells
Priyanka Sharma, et al, Data in Brief Volume 20, October 2018, Pages 1645-1652
Quote: "2H3 cells were cultured overnight in RBL complete medium  on 35 mm petridish containing live cell imaging culture chamber (in vitro Scientific, USA)"
Monitoring of nucleophosmin oligomerization in live cells
A Holoubek,, Methods and Applications in Fluorescence, 2018
Quote: "Live cell imaging The cells were grown on glass bottom Petri dish (Cellvis). Fluorescence experiments were carried out at a room temperature after sealing the Petri dish with parafilm to prevent CO2 leakage"
In vitro irradiation of colorectal cancer cells by pulsed radiation emitted from a hundred joules plasma focus device and its comparison with continuous irradiation
J. Jain et al., IOP Conf. Series: Journal of Physics: Conf. Series 1043 (2018) 012047
Quote: "Immunofluorescence Assay For immunofluorescence assays, 2.4 x 105 cells/well were grown on glass coverslips in 4- chamber 35 mm dish (In vitro Scientific, Sunnyvale, CA, USA)"
P-Rex1 is dispensable for Erk activation and mitogenesis in breast cancer
L Barrio-Real, et al., Oncotarget. 2018 Jun 19; 9(47): 28612–28624.
Quote: "Imaging experiments were carried out in black-walled, 0.17 mm glass-bottom 96-well plates (Cellvis, Mountain View, CA, USA) pre-treated with bovine fibronectin (10 μg/ml in PBS)."