Glass Bottom Dish, Glass Bottom Plate Product FAQs

What are material and thickness of different cover slips?

Cover slip Material Thickness (mm)
#0 Cover glass 0.085-0.115
#1 Cover glass 0.13-0.16
#1.5 Cover glass 0.16-0.19
#1.5H High peformance cover glass 0.170±0.005
#1.5Grid Cover glass with a 20x26 Grid 0.16-0.19

For confocal microscopy, it is recommended to use #1.5H or #1.5 cover glass

What is the spectral transmittance of the cover glass ?

Spectral Transmittance of the #1 cover glass used for our glass bottom dish/plate:

cover glass spectral transmittance

What are the refractive indexes of the cover glass?

ne (λ=546.1 nm):1.5255
nD (λ=589.3 nm):1.5230
Abbe value ve: 55

What kind of adhesive is used to adhere the dish and the cover glass? How can I detach the bottom glass?

We use a USP class VI adhesive to adhere the dish and the cover glass. This adhesive is nontoxic, and it is resistant to water and common fixing reagents such as methanol and ethanol. This makes our glass bottom dishes and glass bottom plates ideal for long term cell culture.

The bad news is that the adherence between the dish and the cover glass is so strong, that it is very difficult to detach the cover glass from the dish.

What is the difference between the plastic surface and glass surface for growing cells?

Most plastic vessels used to culture attached cells are made from polystyrene. Since polystyrene is hydrophobic and most cultured cells can not attach to hydrophobic surface, plastic cell culture vessels are usually surface treated to introduce hydrophilic groups onto the surface to help the attachement of cultured cells.

Glass surface on the other hand, does not need any pre-treatment to allow the attachment of the cultured cells. However the attachment usually is not as strong as that of surface treated polystyrene, our experience indicates that poly-lysine, collagen coating works much better on plastic than on glass. Consequently, poly-lysine or collagen coating on glass are less stable than poly-lysine or collagen coating on surface treated plastics.

What are the exact dimensions of your glass bottom dishes and glass bottom plates?

We have a detailed diagram of each of our glass bottom dishes and glass bottom plates, you can find them in the detailed description of each product. The dimension of the glass bottom plates conforms to ANSI/SBS standard.

What is the harmonized tarrif code of your products?

The harmonized tarrif code of our glass bottom dishes and plates is 3923.90.00