Cellvis: high resolution imaging toolsets for biomedical research.
|Cover slip||Thickness (mm)||Material|
|#1.5H||0.170±0.005||High peformance cover glass|
|#1.5Grid||0.16-0.19||Cover glass with a 20x26 Grid|
|#1.5P||0.175±0.01||Tissue culture treated polymer cover slip with optical characteristics very similar to that of a cover glass.
Recommended for applications where better cell attachment is desired. Not suitable for TIRF.
+: applicable, ++: recommended, Not suitable
|Differential Interference Contrast (DIC)*||+||+||+||+||+||+|
|Two-Photon and Multiphoton Microscopy||+||+||+||+||+||+|
|Fluorescence Recovery After Photobleaching (FRAP)||+||+||+||+||+||+|
|Förster Resonance Energy Transfer (FRET)||+||+||+||+||+||+|
|Fluorescence Lifetime Imaging Microscopy (FLIM)||+||+||+||+||+||+|
|Total Internal Reflection Fluorescence (TIRF)||+||+||+||+||+|
Most tissue culture dishes and plate are made from polystyrene. Polystyrene is hydrophobic by nature, it needs to be tissue culture treated (usually through a physical process using plasma) to render the surface hydrophilic to enable cell attachment.
Typical cover glass has a hydrophilic surface therefore does not require tissue culture treatment to enable cell attachment. However cells ususally attach weaker to cover glass than to tissue culture treated polystyrene dishes and plates. Our experiments show that tissue culture treatment of cover glass does not improve cell attachment.
#1.5P is a tissue culture treated polymer cover slip. It has optical characteristics very similar to that of a cover glass, but shows better attachment for cultured cells than cover glass. In our experience it also shows better attachment for coating media such as poly-lysine and collagen.
The optical property of #1.5P cover slip is:
In comparison with cover glass, #1.5P has high transmittance within UV range (approximately 75% at 280nm). Therefore it is not suitable for applications where UV transparency is not desired.
Because #1.5P is a soft polymer, its flatness is slightly worse than that of a cover glass. This is neglegible for plates with small wells, but may become a problem when the well size gets larger. For this reason we make 12/24/96 well plates with #1.5P, but not 6 well plates.
Overall, our products with #1.5P cover slips are recommended for applications where better cell attachment is desired.
Spectral Transmittance of the #1 cover glass used for our glass bottom dish/plate:
ne (λ=546.1 nm):1.5255
nD (λ=589.3 nm):1.5230
Abbe value ve: 55
We use a USP class VI adhesive to adhere the dish and the cover glass. This adhesive is nontoxic, and it is resistant to water and common fixing reagents such as methanol and ethanol. This makes our glass bottom dishes and glass bottom plates ideal for long term cell culture.
The bad news is that the adherence between the dish and the cover glass is so strong, that it is very difficult to detach the cover glass from the dish.
Most plastic vessels used to culture attached cells are made from polystyrene. Since polystyrene is hydrophobic and most cultured cells can not attach to hydrophobic surface, plastic cell culture vessels are usually surface treated to introduce hydrophilic groups onto the surface to help the attachement of cultured cells.Glass surface on the other hand, does not need any pre-treatment to allow the attachment of the cultured cells. However the attachment usually is not as strong as that of surface treated polystyrene, our experience indicates that poly-lysine, collagen coating works much better on plastic than on glass. Consequently, poly-lysine or collagen coating on glass are less stable than poly-lysine or collagen coating on surface treated plastics.
We have a detailed diagram of each of our glass bottom dishes and glass bottom plates, you can find them in the detailed description of each product. The dimension of the glass bottom plates conforms to ANSI/SBS standard.
The harmonized tarrif code of our glass bottom dishes and plates is 3923.90.00