Cellvis: high resolution imaging toolsets for biomedical research.
|Cover slip||Material||Thickness (mm)|
|#1.5H||High peformance cover glass||0.170±0.005|
|#1.5Grid||Cover glass with a 20x26 Grid||0.16-0.19|
For confocal microscopy, it is recommended to use #1.5H or #1.5 cover glass
Spectral Transmittance of the #1 cover glass used for our glass bottom dish/plate:
ne (λ=546.1 nm):1.5255
nD (λ=589.3 nm):1.5230
Abbe value ve: 55
We use a USP class VI adhesive to adhere the dish and the cover glass. This adhesive is nontoxic, and it is resistant to water and common fixing reagents such as methanol and ethanol. This makes our glass bottom dishes and glass bottom plates ideal for long term cell culture.
The bad news is that the adherence between the dish and the cover glass is so strong, that it is very difficult to detach the cover glass from the dish.
Most plastic vessels used to culture attached cells are made from polystyrene. Since polystyrene is hydrophobic and most cultured cells can not attach to hydrophobic surface, plastic cell culture vessels are usually surface treated to introduce hydrophilic groups onto the surface to help the attachement of cultured cells.Glass surface on the other hand, does not need any pre-treatment to allow the attachment of the cultured cells. However the attachment usually is not as strong as that of surface treated polystyrene, our experience indicates that poly-lysine, collagen coating works much better on plastic than on glass. Consequently, poly-lysine or collagen coating on glass are less stable than poly-lysine or collagen coating on surface treated plastics.
We have a detailed diagram of each of our glass bottom dishes and glass bottom plates, you can find them in the detailed description of each product. The dimension of the glass bottom plates conforms to ANSI/SBS standard.
The harmonized tarrif code of our glass bottom dishes and plates is 3923.90.00