Collagen coating of glass bottom dishes and glass bottom plates


Rat tail collagen (type I) solution

Rat tail collagen I is available from many commercial vendors, such as BD Biosciences(cat# 354249), Sigma (cat #C3867), Invitrogen (cat #A10483-01).

It is recommended to purchase collagen solutions, so that sterilization is not needed since filter sterilization of collagen could be problematic.

Dilution the stock solution to 50 ug/ml with 0.02M acetic acid or 0.01M HCl.


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