Fibronectin coating of glass bottom dishes and glass bottom plates


Fibronectin solution

Human Fibronectin from BD Biosciences(cat# 354008) is diluted to 10-50ug/ml with Ca++ and Mg++ free PBS. The concentration should be proportional to coating concentration, i.e, make 10ug/ml solution for 1ug/cm2 coating, 50ug/ml for 5ug/cm2 coating, etc.

Make appropriate aliquots and store at -20℃.

Do not store in frost-free freezer, avoid multiple freeze thaws.


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