Poly-lysine coating of glass bottom dishes and glass bottom plates


Poly-lysine solution

Both poly-L-lysine and poly-D-lysine of MW between 30,000 - 150,000 can be used for coating. Poly-D-lysine is recommended since it is not subjected to protease released from cells.

Prepare 1mg/ml stock solution by dissolving 100mg Poly-D-lysine hydrobromide(Sigma P-0899) in 100 ml water. Filter sterilize the solution then store aliquots at –20℃.

Working solution is obtained by diluting the stock solution with sterile water to around 0.1mg/ml (0.05-0.5mg/ml).


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